In vitro removal of human IgG autoantibodies by affinity filtration using immobilized L-histidine onto PEVA hollow fiber membranes

R C Ventura; R L Zollner; C Legallais; M Vijayalakshmi; S M Bueno

doi:10.1016/s1389-0344(00)00069-1

In vitro removal of human IgG autoantibodies by affinity filtration using immobilized L-histidine onto PEVA hollow fiber membranes

Biomol Eng. 2001 Jan;17(2):71-4. doi: 10.1016/s1389-0344(00)00069-1.

Authors

R C Ventura¹, R L Zollner, C Legallais, M Vijayalakshmi, S M Bueno

Affiliation

¹ School of Chemical Engineering, State University of Campinas, UNICAMP, SP, Campinas, Brazil.

PMID: 11163754
DOI: 10.1016/s1389-0344(00)00069-1

Abstract

Histidine was immobilized onto PEVA membrane to obtain an affinity support for human IgG removal from serum with a view to clinical apheresis for the treatment of autoimmune diseases. These membranes were able to remove in vitro several autoantibodies from the serum of SLE patients.

MeSH terms

Amino Acids / chemistry
Antibodies / isolation & purification
Filtration / methods*
Histidine / chemistry*
Humans
Immunoglobulin G / blood
Immunoglobulin G / immunology*
Immunoglobulin G / isolation & purification*
Lupus Erythematosus, Systemic / blood
Lupus Erythematosus, Systemic / immunology
Lupus Erythematosus, Systemic / therapy
Polyvinyls / chemistry*

Substances

Amino Acids
Antibodies
Immunoglobulin G
Polyvinyls
ethylene-vinyl alcohol copolymer
Histidine