Characterization of the common nodulation genes of the photosynthetic Bradyrhizobium sp. ORS285 reveals the presence of a new insertion sequence upstream of nodA

FEMS Microbiol Lett. 2001 Jan 1;194(1):83-6. doi: 10.1111/j.1574-6968.2001.tb09450.x.

Abstract

We isolated and characterized nodA genes from photosynthetic and non-photosynthetic rhizobia nodulating the legume genus Aeschynomene, and found that the nodA sequence from photosynthetic stem-nodulating bacteria was phylogenetically distant from the other already described nodA genes. Characterization of the photosynthetic strain ORS285 common nod gene cluster (nodABC) showed, upstream of nodA, the presence of a new insertion sequence element belonging to the IS3 family and specific to a group of photosynthetic strains from Aeschynomene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyltransferases / genetics*
  • Bacterial Proteins
  • Bradyrhizobium / genetics*
  • Bradyrhizobium / physiology
  • DNA Transposable Elements*
  • Fabaceae / microbiology
  • Genes, Bacterial*
  • Genomic Library
  • Molecular Sequence Data
  • Multigene Family
  • Nitrogen Fixation / genetics*
  • Photosynthesis / physiology*
  • Phylogeny
  • Plants, Medicinal
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins
  • DNA Transposable Elements
  • Acyltransferases
  • NodA protein, Rhizobiales

Associated data

  • GENBANK/AF284858