Abstract
WHSC2, a novel gene recently isolated within the critical region of Wolf-Hirschhorn syndrome, is expressed in endothelial cells. WHSC2 is downregulated by HIV-1 Tat, whereas it is not modulated by angiogenic and pro-differentiative molecules. WHSC2 encodes a basic polypeptide of 528 amino acids. The in vitro translated protein shows a molecular weight of 57 kDa. WHSC2 has two nuclear translocation sequences which actively mediate its transport to the nucleus, as shown in whsc2-GFP-transfected NIH-3T3. We also found a helix-loop-helix (HLH) motif in region 130-185. Since members of the HLH family control differentiation and cell cycle progression, we hypothesize that WHSC2 may function as a transcriptional repressor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Cell Cycle
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Cell Differentiation
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Cell Nucleus / metabolism
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Cells, Cultured
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Endothelial Growth Factors / pharmacology
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Endothelium, Vascular / cytology
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Endothelium, Vascular / physiology*
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Fibroblast Growth Factor 2 / pharmacology
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / physiology*
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Gene Products, tat / pharmacology
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Genes, Reporter
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Green Fluorescent Proteins
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Growth Substances / pharmacology*
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HIV-1
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Helix-Loop-Helix Motifs
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Hepatocyte Growth Factor / pharmacology
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Humans
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Luminescent Proteins / genetics
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Lymphokines / pharmacology
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Mice
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Molecular Sequence Data
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Protein Biosynthesis
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Protein Structure, Secondary
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Protein Transport
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Proteins / chemistry
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Proteins / genetics*
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Proteins / metabolism*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription, Genetic
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Transcriptional Elongation Factors
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Transfection
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Umbilical Veins
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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tat Gene Products, Human Immunodeficiency Virus
Substances
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Endothelial Growth Factors
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Gene Products, tat
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Growth Substances
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Luminescent Proteins
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Lymphokines
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NELFA protein, human
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Proteins
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Recombinant Proteins
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Transcriptional Elongation Factors
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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tat Gene Products, Human Immunodeficiency Virus
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Fibroblast Growth Factor 2
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Green Fluorescent Proteins
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Hepatocyte Growth Factor