Effect of airflow rate on yields of Steinernema carpocapse Az 20 in liquid culture in an external-loop airlift bioreactor

Biotechnol Bioeng. 2001 Feb 5;72(3):369-73. doi: 10.1002/1097-0290(20010205)72:3<369::aid-bit15>3.0.co;2-f.

Abstract

Maximization of the contact between males and females is a key factor in the production of the nematode Steinernema carpocapsae in a bioreactor.%The influence of the airflow rate in male and female distribution and mass production in an external-loop bioreactor with a deceleration zone was studied. When operating at an airflow rate of 0.05 vvm, a high retention of females in the deceleration zone of the bioreactor was observed and a larger nematode productivity was obtained. At this aeration rate there was a higher proportion of males in that zone, which together with the lower circulation rate, increases the probability of encounters, thereby explaining the increase in productivity.

MeSH terms

  • Air Movements*
  • Animals
  • Bioreactors*
  • Female
  • Insecta / growth & development
  • Insecta / microbiology
  • Insecta / parasitology
  • Lethal Dose 50
  • Male
  • Pest Control, Biological
  • Reproduction
  • Rhabditida / growth & development*
  • Rhabditida / microbiology
  • Rhabditida / physiology*
  • Xenorhabdus / physiology