Effect of various environmental parameters on the recovery of sublethally salt-damaged and acid-damaged Listeria monocytogenes

J Appl Microbiol. 2000 Dec;89(6):944-50. doi: 10.1046/j.1365-2672.2000.01195.x.

Abstract

The influence of supplementing the culture medium with magnesium sulphate, D-glucose, L-cysteine, catalase or lithium chloride, of incubation temperature and of oxygen availability on the recovery of salt- or acid-damaged Listeria monocytogenes, was studied on a solid repair medium according to a Hadamard matrix, with seven parameters varying between a high and a low level. The most important factors for repair of stressed Listeria were further studied with complete factorial design experiments. Results show that conditions promoting resuscitation of acid- or salt-injured cells are stress-specific, and differ in part from those described in the literature for heat-stressed Listeria.

MeSH terms

  • Anaerobiosis
  • Catalase / pharmacology
  • Culture Media
  • Cysteine / pharmacology
  • Glucose / pharmacology
  • Hydrogen-Ion Concentration
  • Listeria monocytogenes / drug effects
  • Listeria monocytogenes / growth & development
  • Listeria monocytogenes / physiology*
  • Lithium Chloride / pharmacology
  • Magnesium Sulfate / pharmacology
  • Regression Analysis
  • Sodium Chloride / pharmacology*
  • Temperature

Substances

  • Culture Media
  • Sodium Chloride
  • Magnesium Sulfate
  • Catalase
  • Lithium Chloride
  • Glucose
  • Cysteine