Abstract
Enteropathogenic Escherichia coli (EPEC) strain 30-5-1(3) has been reported to form attaching and effacing (A/E) lesions without Tir tyrosine phosphorylation. In this study, we show that 30-5-1(3), which has a transposon insertion within the sepZ gene, forms wild-type A/E lesions including Tir tyrosine phosphorylation, but at a slower rate. A/E lesion formation by 30-5-1(3) occurs without detectable secretion of Tir or other EPEC Esp secreted proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Adhesion
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Bacterial Proteins / analysis
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Bacterial Proteins / genetics*
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Bacterial Proteins / physiology
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DNA Transposable Elements*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Escherichia coli / pathogenicity*
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Escherichia coli Proteins*
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HeLa Cells
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Humans
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Mutation
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Phosphorylation
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Receptors, Cell Surface / metabolism*
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Tyrosine / metabolism*
Substances
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Bacterial Proteins
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DNA Transposable Elements
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Escherichia coli Proteins
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EspA protein, E coli
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Receptors, Cell Surface
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SepZ protein, E coli
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Tir protein, E coli
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Tyrosine