Cloning and expression of cDNAs encoding two enzymes of the MEP pathway in Catharanthus roseus

Biochim Biophys Acta. 2000 Dec 15;1517(1):159-63. doi: 10.1016/s0167-4781(00)00240-2.

Abstract

Two periwinkle cDNAs (crdxr and crmecs) encoding enzymes of the non-mevalonate terpenoid pathway were characterized using reverse transcription-PCR strategy based on the design of degenerated oligonucleotides. The deduced amino acid sequence of crdxr is homologue to 1-deoxy-D-xylulose 5-phosphate reductoisomerases. Crmecs represents the first plant cDNA encoding a protein similar to the 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Escherichia coli. Expression of crdxr and crmecs genes was up-regulated in periwinkle cells producing monoterpenoid indole alkaloids. Involvement of the 2C-methyl-D-erythritol 4-phosphate pathway in alkaloid biosynthesis is discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldose-Ketose Isomerases / chemistry
  • Aldose-Ketose Isomerases / genetics*
  • Alkaloids
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / chemistry*
  • Erythritol / analogs & derivatives
  • Erythritol / metabolism*
  • Genes, Plant*
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry
  • Multienzyme Complexes / genetics*
  • Nucleotidyltransferases / chemistry
  • Nucleotidyltransferases / genetics*
  • Oxidoreductases / chemistry
  • Oxidoreductases / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sugar Phosphates / metabolism*

Substances

  • 2-C-methylerythritol 4-phosphate
  • Alkaloids
  • DNA, Complementary
  • Multienzyme Complexes
  • Sugar Phosphates
  • Oxidoreductases
  • 1-deoxy-D-xylulose 5-phosphate reductoisomerase
  • 4-diphosphocytidyl-2-C--methylerythritol synthase
  • Nucleotidyltransferases
  • Aldose-Ketose Isomerases
  • Erythritol

Associated data

  • GENBANK/AF250235
  • GENBANK/AF250236