Abstract
A (1 --> 3)-beta-D-glucan with approximately 30% of the residues having a beta-D-Glc-(1 --> 6) branch is the main water-soluble component of the cell wall polysaccharide of Cryphonectria parasitica (Murr.) Barr strain 263. A (1 --> 3)-glucan with both alpha and beta anomeric linkages was found in the water-insoluble polysaccharide fraction. Both fractions possess immunological activity, being able to induce the production of either tumour necrosis factor alpha (TNF-alpha) or nitrite (NO2-).
MeSH terms
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Adjuvants, Immunologic / chemistry
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Adjuvants, Immunologic / isolation & purification*
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Animals
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Ascomycota / chemistry*
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Carbohydrate Conformation
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Carbohydrate Sequence
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Cell Wall / chemistry*
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Enzyme Induction
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Glucans / chemistry
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Glucans / isolation & purification*
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Glucans / pharmacology
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Lipopolysaccharides / pharmacology
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Macrophages / metabolism
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Magnetic Resonance Spectroscopy
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Mice
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Molecular Sequence Data
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Neoplasm Proteins / metabolism
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Nitric Oxide Synthase / metabolism
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Nitric Oxide Synthase Type II
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Nitrites / metabolism
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Polysaccharides / analysis
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Solubility
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Structure-Activity Relationship
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Tumor Cells, Cultured
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Tumor Necrosis Factor-alpha / metabolism
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Water
Substances
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Adjuvants, Immunologic
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Glucans
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Lipopolysaccharides
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Neoplasm Proteins
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Nitrites
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Polysaccharides
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Tumor Necrosis Factor-alpha
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Water
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse