Immunostimulant (1 --> 3)-D-glucans from the cell wall of Cryphonectria parasitica (Murr.) Barr strain 263

Carbohydr Res. 2000 Nov 3;329(2):441-5. doi: 10.1016/s0008-6215(00)00178-6.

Abstract

A (1 --> 3)-beta-D-glucan with approximately 30% of the residues having a beta-D-Glc-(1 --> 6) branch is the main water-soluble component of the cell wall polysaccharide of Cryphonectria parasitica (Murr.) Barr strain 263. A (1 --> 3)-glucan with both alpha and beta anomeric linkages was found in the water-insoluble polysaccharide fraction. Both fractions possess immunological activity, being able to induce the production of either tumour necrosis factor alpha (TNF-alpha) or nitrite (NO2-).

Publication types

  • Comparative Study

MeSH terms

  • Adjuvants, Immunologic / chemistry
  • Adjuvants, Immunologic / isolation & purification*
  • Animals
  • Ascomycota / chemistry*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cell Wall / chemistry*
  • Enzyme Induction
  • Glucans / chemistry
  • Glucans / isolation & purification*
  • Glucans / pharmacology
  • Lipopolysaccharides / pharmacology
  • Macrophages / metabolism
  • Magnetic Resonance Spectroscopy
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins / metabolism
  • Nitric Oxide Synthase / metabolism
  • Nitric Oxide Synthase Type II
  • Nitrites / metabolism
  • Polysaccharides / analysis
  • Solubility
  • Structure-Activity Relationship
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / metabolism
  • Water

Substances

  • Adjuvants, Immunologic
  • Glucans
  • Lipopolysaccharides
  • Neoplasm Proteins
  • Nitrites
  • Polysaccharides
  • Tumor Necrosis Factor-alpha
  • Water
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse