Abstract
Venezuelan equine encephalitis virus replicon particles (VRP) containing the gene expressing hemagglutinin (HA) from the human Hong Kong Influenza A isolate (A/HK/156/97) were evaluated as vaccines in chicken embryos and young chicks. Expressed HA was readily detected in bird-tissue staining with anti-H5 HA antibody and in chicken cells infected with the replicon preparations following immunoprecipitation with monoclonal antibody. Birds challenged with a dose of the lethal parent virus were protected to different extents depending on the age of the bird. In ovo and 1-day-old inoculated animals that received no boost with the VRP were partially protected; birds 2 weeks of age were completely protected with a single dose of VRP.
Copyright 2000 Academic Press.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Age Factors
-
Animals
-
Antibodies, Monoclonal
-
Antigens, Viral / analysis
-
Antigens, Viral / genetics*
-
Antigens, Viral / immunology
-
Cells, Cultured
-
Chick Embryo
-
Chickens
-
Encephalitis Virus, Venezuelan Equine / genetics*
-
Genetic Vectors
-
Hemagglutinin Glycoproteins, Influenza Virus / analysis
-
Hemagglutinin Glycoproteins, Influenza Virus / genetics*
-
Hemagglutinin Glycoproteins, Influenza Virus / immunology
-
Humans
-
Immunohistochemistry
-
Influenza A Virus, H5N1 Subtype*
-
Influenza A virus / immunology*
-
Influenza, Human / prevention & control*
-
Influenza, Human / veterinary
-
Influenza, Human / virology
-
Poultry Diseases / prevention & control
-
Replicon / genetics*
-
Vaccination
-
Vaccines, Synthetic / administration & dosage*
-
Viral Vaccines / administration & dosage*
Substances
-
Antibodies, Monoclonal
-
Antigens, Viral
-
Hemagglutinin Glycoproteins, Influenza Virus
-
Vaccines, Synthetic
-
Viral Vaccines