Chromosome 2 short arm translocations revealed by M-FISH analysis of neuroblastoma cell lines

N Van Roy; H Van Limbergen; J Vandesompele; M Van Gele; B Poppe; G Laureys; A De Paepe; F Speleman

doi:10.1002/1096-911x(20001201)35:6<538::aid-mpo8>3.0.co;2-c

Chromosome 2 short arm translocations revealed by M-FISH analysis of neuroblastoma cell lines

Med Pediatr Oncol. 2000 Dec;35(6):538-40. doi: 10.1002/1096-911x(20001201)35:6<538::aid-mpo8>3.0.co;2-c.

Authors

N Van Roy¹, H Van Limbergen, J Vandesompele, M Van Gele, B Poppe, G Laureys, A De Paepe, F Speleman

Affiliation

¹ Center of Medical Genetics, Ghent University Hospital, Gent, Belgium.

PMID: 11107111
DOI: 10.1002/1096-911x(20001201)35:6<538::aid-mpo8>3.0.co;2-c

Abstract

Procedure: M-FISH analysis was performed on 18 neuroblastoma cell lines, which were previously studied with cytogenetic, standard FISH and CGH data.

Results: One of the most striking findings of this study was the detection of chromosome 2 short arm rearrangements in 61% of the investigated cell lines. These rearrangements resulted from translocations with various partner chromosomes. All translocations, except one were unbalanced, leading to the consistent gain of chromosome segment 2pter-p22. A cryptic balanced translocation t(2;4) was observed with a breakpoint located in the vicinity of MYCN in cell line NBL-S.

Conclusions: Combination of M-FISH results together with cytogenetic, standard FISH and CGH data yielded the most comprehensive description of chromosome 2 short arm rearrangements, leading to a consistent gain of chromosome 2 short arm material.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Child
Chromosomes, Human, Pair 2 / genetics*
Humans
In Situ Hybridization, Fluorescence
Neuroblastoma / genetics*
Translocation, Genetic / genetics*
Tumor Cells, Cultured