Abstract
The mouse disabled 2 (mDab2) gene is a mouse homologue of the Drosophila disabled gene and is alternatively spliced to form two isoforms, p96 and p67. Although p96 has been known to regulate the Ras-Sos G-protein signal transduction pathway by interacting with Grb2, little is known about the biological function of p67. Recent studies have shown that the expression of mDab2 is markedly up-regulated during the retinoic acid (RA)-induced differentiation of F9 cells, suggesting another role for mDab2 in cell differentiation [Cho, Lee and Park (1999) Mol. Cells 9, 179-184). In the present study, we first elucidated the biological function of p67 isoform of mDab2 and identified its binding partner. Unlike p96, p67 largely resides in RA-treated F9 cell nuclei. In this system, p67 interacts with mouse androgen-receptor interacting protein 3, termed the mDab2 interacting protein, which acts as a transcriptional co-regulator. By using a fusion protein with a heterologous DNA-binding domain (GAL4), we showed that p67 had an intrinsic transcriptional activation function. These results suggest that mDab2 p67 may function as a transcriptional co-factor for certain complexes of transcriptional regulatory elements involved in the RA-induced differentiation of F9 cells.
MeSH terms
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3T3 Cells
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport*
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Animals
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Apoptosis Regulatory Proteins
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Binding Sites
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COS Cells
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Cell Differentiation* / drug effects
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Cell Line
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Cell Nucleus / chemistry
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Cell Nucleus / drug effects
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Cell Nucleus / metabolism
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DNA-Binding Proteins
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Genes, Tumor Suppressor
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Mice
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Molecular Sequence Data
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Molecular Weight
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Nuclear Proteins / chemistry
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Phosphoproteins / chemistry
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Precipitin Tests
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Protein Binding
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Protein Inhibitors of Activated STAT
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Proteins*
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Rats
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Trans-Activators / chemistry
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transcriptional Activation
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Transfection
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Tretinoin / pharmacology
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Tumor Suppressor Proteins
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Two-Hybrid System Techniques
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Ubiquitin-Protein Ligases
Substances
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport
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Apoptosis Regulatory Proteins
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DAB2 protein, human
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DNA-Binding Proteins
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Nuclear Proteins
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Phosphoproteins
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Protein Inhibitors of Activated STAT
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Protein Isoforms
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Proteins
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Recombinant Fusion Proteins
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Trans-Activators
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Tumor Suppressor Proteins
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Tretinoin
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Miz1 protein, mouse
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Ubiquitin-Protein Ligases
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Pias2 protein, rat