Quantitation of spermatogenesis by DNA flow cytometry from fine-needle aspiration cytology material

P Dey; A K Mondal; S K Singh; H Vohra

doi:10.1002/1097-0339(200012)23:6<386::aid-dc4>3.0.co;2-s

Quantitation of spermatogenesis by DNA flow cytometry from fine-needle aspiration cytology material

Diagn Cytopathol. 2000 Dec;23(6):386-7. doi: 10.1002/1097-0339(200012)23:6<386::aid-dc4>3.0.co;2-s.

Authors

P Dey¹, A K Mondal, S K Singh, H Vohra

Affiliation

¹ Department of Cytology, Post Graduate Institute of Medical Education and Research, Chandigarh, India. pranab@ch1.dot.net.in

PMID: 11074642
DOI: 10.1002/1097-0339(200012)23:6<386::aid-dc4>3.0.co;2-s

Abstract

DNA flow cytometry (FCM) was performed from fine-needle aspiration cytology (FNAC) of testis in 15 cases of male infertility to quantitate spermatogenesis. The results were correlated with FNAC findings. DNA FCM showed a ploidy relationship of haploid (1N) > diploid (2N) > tetraploid (4N) in cases of normal spermatogenesis. A ploidy relation of 2N > 1N > 4N was observed in cases of hypospermatogenesis or maturation arrest. In Sertoli cell-only cases, there were only 2N populations of cells. With the help of DNA FCM, a rapid and objective assessment of spermatogenesis is possible from FNAC of the testis.

MeSH terms

Biopsy, Needle
DNA / analysis*
Flow Cytometry / methods
Humans
Infertility, Male / genetics*
Infertility, Male / pathology
Male
Oligospermia / genetics
Oligospermia / pathology
Ploidies
Sertoli Cells / pathology
Spermatogenesis / genetics*
Syndrome
Testis / pathology*

Substances

DNA