Abstract
The genomic sequence of the human GCMa/GCM1 gene, a mammalian homologue of Drosophila melanogaster GCM, was determined. Drosophila GCM is a neural transcription factor that regulates glial cell fate. The mammalian homolog however, is a placenta-specific transcription factor that is necessary for placental development. The 22 kb DNA sequence spanning the GCMa gene contains six exons and five introns, encoding a 2.8 kb cDNA. Overall genomic organization is similar for the human and mouse. Several potential binding sites for transcription factors like GATA, Oct-1, and bHLH proteins were found in the 5'-flanking region of the human gene. A DNA motif for GCM protein binding exists in the 5'-flanking region that is highly homologous with that of the mouse gene. The location of this gene was mapped to chromosome 6 using fluorescence in situ hybridization.
Copyright 2000 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Basic Helix-Loop-Helix Transcription Factors
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Binding Sites
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Chromosome Mapping
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Chromosomes, Human, Pair 6
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DNA, Complementary / genetics
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DNA-Binding Proteins / metabolism
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Exons
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GATA2 Transcription Factor
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GATA3 Transcription Factor
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Gene Library
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Host Cell Factor C1
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Humans
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In Situ Hybridization, Fluorescence
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Introns
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Mice
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Models, Genetic
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Molecular Sequence Data
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Neuropeptides / genetics*
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Nuclear Proteins
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Octamer Transcription Factor-1
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Placenta / metabolism*
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Polymerase Chain Reaction
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Sequence Homology, Nucleic Acid
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Trans-Activators / metabolism
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Transcription Factors / metabolism
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Trophoblasts
Substances
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Basic Helix-Loop-Helix Transcription Factors
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DNA, Complementary
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DNA-Binding Proteins
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GATA2 Transcription Factor
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GATA2 protein, human
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GATA3 Transcription Factor
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GATA3 protein, human
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GCM1 protein, human
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Gata2 protein, mouse
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Gata3 protein, mouse
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Gcm1 protein, mouse
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HCFC1 protein, human
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Hcfc1 protein, mouse
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Host Cell Factor C1
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Neuropeptides
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Nuclear Proteins
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Octamer Transcription Factor-1
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POU2F1 protein, human
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Pou2f1 protein, mouse
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Trans-Activators
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Transcription Factors