Objective: To investigate the effectiveness of recombinant retrovirus vector in gene therapy.
Methods: The retroviral vector PLXSN-S was constructed and transferred into PA317 by means of electroporation, then HepG(2), P815, and EL4 cells were infected with the pseudovirus produced from PA317, which highly expressed HBsAg. HBsAg expression was tested by RT-PCR and ELISA.
Results: HBsAg was expressed variously in the eukaryotic cells mentioned above. HBsAg (A value) of the cell supernatants (48 h) were 0.92, 0.09, 0.47, respectively.
Conclusion: The vector used in this study is an effective one to carry genes of interest to target cells and it may be useful in the test for gene therapy.