Loss of vitreous volume as a result of injury or surgical intervention leads to death of the eye. The authors developed a method for hyalocyte culturing on the glass in culture medium. Culture medium was as follows (100 ml): 50 ml medium 199, 30 ml lactate albumin hydrolysate, and 20 ml human serum, group IV. Vitreous cells were collected from rabbit eyes. Primary and secondary growth zones were distinguished in the course of culture growth. Morphologically intact rabbit hyalocytes in the culture were polymorphous. These results may indicate a possibility of transplanted culture development in a live eye.