Abstract
Alterations in gene expression during early stages of dormancy release in grapevine buds were analyzed to facilitate the identification of gene products that may mediate the signal transduction of a dormancy-release signal, or derepression of meristematic activity. In the present report we describe the identification of GDBRPK, a transcript for an SNF-like protein kinase that is up-regulated upon chemical induction of dormancy release by hydrogen cyanamide (HC). Since SNF and SNF-like protein kinases are known as sensors of stress signals, we hypothesize that GDBRPK may be involved in the perception of a stress signal induced by HC. We also describe a simultaneous and remarkable induction of both PDC and ADH transcripts that was observed shortly after HC application, and was of a transient nature. These data may imply that HC application leads to a transient respiratory stress, which likely results in a temporary increase in the AMP/ATP ratio. Since AMP is known as a stress signal that is sensed by SNF-like kinases, we suggest that the SNF-like GDBRPK could serve as the sensor of this signal.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Dehydrogenase / drug effects
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Alcohol Dehydrogenase / metabolism
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Amino Acid Sequence
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Blotting, Northern
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Blotting, Southern
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Cloning, Molecular
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Cyanamide / pharmacology*
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA, Plant / genetics
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Fermentation / drug effects
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Gene Expression Regulation, Developmental / drug effects
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Plant / drug effects
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Molecular Sequence Data
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Protein Serine-Threonine Kinases / genetics*
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Protein Serine-Threonine Kinases / metabolism
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Pyruvate Decarboxylase / drug effects
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Pyruvate Decarboxylase / metabolism
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RNA, Plant / drug effects
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Rosales / drug effects*
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Rosales / genetics
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Rosales / growth & development
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Signal Transduction / drug effects*
Substances
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DNA, Complementary
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DNA, Plant
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RNA, Plant
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Cyanamide
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Alcohol Dehydrogenase
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Protein Serine-Threonine Kinases
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Pyruvate Decarboxylase