Comparative molecular analysis of erythromycin-resistance determinants in staphylococcal isolates of poultry and human origin

Mol Cell Probes. 2000 Oct;14(5):311-9. doi: 10.1006/mcpr.2000.0320.

Abstract

The ermA, ermB, ermC and msrA/msrB genes were detected in multidrug-resistant Staphylococcus spp. strains by PCR. Among 25 human clinical staphylococcal isolates the ermA, ermB, ermC and the msrA/msrB genes were detected in 88, 72, 4 and 100% of the strains, respectively. Among 24 poultry isolates the ermA, ermB, ermC and the msrA/msrB genes were detected in 100, 16.6, 50 and 12.5% of the strains, respectively. The ermA gene was found exclusively on the chromosome, whereas the ermC gene was found on 2.4-4.2 kb plasmids. Restriction fragment length polymorphism (RFLP) analysis of the ermA gene with Eco RI revealed five patterns (25.0, 21.0, 10.5, 6.2 and 4. 8 kb) for the clinical strains and two (8.0 and 6.2 kb) for the poultry strains. The 6.2 kb RFLP pattern, in both the poultry and human clinical isolates, indicates a common lineage for the ermA gene.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Deoxyribonuclease EcoRI / genetics
  • Drug Resistance, Microbial / genetics*
  • Drug Resistance, Multiple / genetics
  • Erythromycin / pharmacology*
  • Gene Dosage
  • Genetic Markers
  • Humans
  • Membrane Transport Proteins*
  • Methyltransferases / genetics
  • Microbial Sensitivity Tests
  • Plasmids / genetics
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length
  • Poultry / microbiology*
  • Staphylococcus / drug effects*
  • Staphylococcus / physiology*

Substances

  • Bacterial Proteins
  • Genetic Markers
  • Membrane Transport Proteins
  • msrA protein, Staphylococcus epidermidis
  • Erythromycin
  • Methyltransferases
  • rRNA (adenosine-O-2'-)methyltransferase
  • Deoxyribonuclease EcoRI