Abstract
The present study evaluated four chimeric synthetic peptides incorporating immunodominant sequences from HTLV-1 virus. Monomeric peptides M1, M2, and M3 represent sequences from core (p19) and envelope (gp46) of the virus. The peptide M1 is a p19 (105-124) sequence, the peptide M2 is a gp46 (190-207) sequence, and the peptide M3 is a gp 46 sequence with substitution of proline at position 192 by serine. Those peptides were arranged in such a way that permits one to obtain different combinations of chimeric peptides (M1-M2, M2-M1, M1-M3, and M3-M1). Two glycine residues were used as arm spacers for separating the two sequences. The antigenicity of these peptides was evaluated in an ultramicroenzyme-linked immunosorbent assay (UMELISA) using sera of human T cell leukemia virus type I (HTLV-I)-infected individuals (n = 24), while specificity was evaluated with anti-HTLV-II-positive samples (n = 11) and healthy blood donors (n = 25). The results were compared to plates coated with monomeric peptides M1, M2, and M3. The chimeric peptide orientation (M1-M2) and the proline at position 192 of the gp46 peptide showed higher sensitivity.
Copyright 2000 Academic Press.
MeSH terms
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Amino Acid Sequence
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Antibody Specificity
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Enzyme-Linked Immunosorbent Assay
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Gene Products, env / chemistry
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Gene Products, env / immunology
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Gene Products, gag / chemistry
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Gene Products, gag / immunology
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HTLV-I Antigens / chemistry
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HTLV-I Antigens / immunology*
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HTLV-II Antigens / immunology
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Human T-lymphotropic virus 1 / chemistry
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Human T-lymphotropic virus 1 / immunology*
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Human T-lymphotropic virus 2 / immunology
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Humans
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Immune Sera / immunology
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Immunodominant Epitopes / chemistry*
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Immunodominant Epitopes / immunology*
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Molecular Sequence Data
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Peptides / chemical synthesis
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Peptides / chemistry
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Peptides / immunology
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Protein Conformation
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Recombinant Fusion Proteins / chemical synthesis
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Recombinant Fusion Proteins / chemistry*
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Recombinant Fusion Proteins / immunology*
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Retroviridae Proteins, Oncogenic / chemistry
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Retroviridae Proteins, Oncogenic / immunology
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Sensitivity and Specificity
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gag Gene Products, Human Immunodeficiency Virus
Substances
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Gene Products, env
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Gene Products, gag
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HTLV-I Antigens
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HTLV-II Antigens
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Immune Sera
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Immunodominant Epitopes
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Peptides
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Recombinant Fusion Proteins
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Retroviridae Proteins, Oncogenic
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gag Gene Products, Human Immunodeficiency Virus
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gp46 protein, Human T-cell leukemia virus type I
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p19 protein, Human T-lymphotropic virus 1