Abstract
New chemistry for the fluorescent labeling of oligonucleotides with cyanine dyes is proposed. It is based on the use of pyrylium salts as amine-specific reagents. Monomethyne pyrylium cyanine dye 1 was covalently linked to 5'-aminoalkyl modified oligonucleotide, with simultaneous conversion of the non-fluorescent dye 1 into fluorescent pyridinium cyanine structure 2.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amines / chemistry
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Chromatography, High Pressure Liquid
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism
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Molecular Structure
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Oligonucleotides / chemistry*
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Oligonucleotides / metabolism
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Staining and Labeling / methods*
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Thiazoles / chemistry*
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Thiazoles / metabolism
Substances
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Amines
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Cyan 39
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Fluorescent Dyes
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Oligonucleotides
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Thiazoles