Abstract
The human cytochrome c(1) promoter is strongly activated in transfected Drosophila SL2 cells expressing exogenous human E2F1. Transfection-deletion experiments, DNase I protection by E2F1 and gel mobility-shift experiments locate E2F1 activation sites to two regions on either side of the transcription start site. Deletion of either region prevents E2F1 activation in transfected SL2 cells, suggesting a co-operative interaction between them. E2F6, a member of the E2F family that lacks transactivation domains but contains specific suppressor domains, inhibits cytochrome c(1) promoter activity when co-transfected into HeLa cells, indicating that the E2F proteins modulate the cytochrome c(1) promoter in mammalian cells. However, E2F is not a general regulator of oxidative phosphorylation genes since three additional nuclear-encoded mitochondrial genes were unaffected by E2F1 or E2F6.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Carrier Proteins*
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Cell Cycle Proteins*
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Cell Line
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Cell Nucleus / genetics
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Cytochromes c1 / genetics*
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DNA / genetics
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DNA / metabolism
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DNA Footprinting
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Deoxyribonuclease I / metabolism
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Drosophila Proteins*
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Drosophila melanogaster
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E2F Transcription Factors
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E2F1 Transcription Factor
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E2F6 Transcription Factor
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Gene Expression Regulation*
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HeLa Cells
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Humans
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Mitochondria / metabolism
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Oxidative Phosphorylation
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Promoter Regions, Genetic / genetics*
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Protein Structure, Tertiary
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Repressor Proteins / chemistry
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Response Elements / genetics
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Retinoblastoma-Binding Protein 1
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Sequence Deletion / genetics
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Substrate Specificity
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Trans-Activators*
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Transcription Factor DP1
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transfection
Substances
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Carrier Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins
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Dp transcription factor, Drosophila
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Drosophila Proteins
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E2F Transcription Factors
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E2F1 Transcription Factor
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E2F1 protein, human
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E2F6 Transcription Factor
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E2F6 protein, human
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Repressor Proteins
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Retinoblastoma-Binding Protein 1
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Trans-Activators
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Transcription Factor DP1
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Transcription Factors
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DNA
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Cytochromes c1
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Deoxyribonuclease I