Abstract
We report the cloning and sequencing of the Brucella abortus oxyR homolog and provide evidence that the transcription product of this gene binds to the B. abortus catalase promoter region. A gene replacement/deletion Brucella oxyR mutant exhibits increased sensitivity to prolonged exposure to H(2)O(2) and is unable to adapt to H(2)O(2) in the environment.
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / physiology*
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Base Sequence
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Brucella abortus / drug effects
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Brucella abortus / genetics
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Brucella abortus / metabolism*
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Catalase / genetics*
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DNA, Bacterial
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DNA-Binding Proteins*
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Gene Expression Regulation, Enzymologic*
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Hydrogen Peroxide / pharmacology
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Molecular Sequence Data
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Promoter Regions, Genetic
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Repressor Proteins / genetics
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Repressor Proteins / physiology*
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Transcription Factors / genetics
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Transcription Factors / physiology*
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA-Binding Proteins
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Repressor Proteins
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Transcription Factors
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Hydrogen Peroxide
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Catalase