Laser irradiative tissue probed in situ by collagen 380-nm fluorescence imaging

Lasers Surg Med. 2000;27(2):158-64. doi: 10.1002/1096-9101(2000)27:2<158::aid-lsm7>3.0.co;2-i.

Abstract

Background and objective: There is no ideal method to detect molecular changes in situ of laser-irradiated tissue without removing a section of tissue for histologic examination. A new method is reported to evaluate laser-induced thermal tissue damage in situ by using 380-nm native fluorescence imaging under 340-nm excitation.

Study design/materials and methods: Native fluorescence imaging was performed on laser irradiated bovine tendon tissue and compared with light illuminated photography and histology with picrosirius red F3BA stain.

Results and conclusion: The results indicate that a decrease in collagen fluorescence at 380 nm was observed in laser-induced thermally damaged tissues. The injured region of tissue defined by its fluorescence image coincided with an area defined by photography and histology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Azo Compounds
  • Burns / diagnosis*
  • Burns / etiology
  • Burns / pathology
  • Cattle
  • Coloring Agents
  • Hot Temperature / adverse effects*
  • In Vitro Techniques
  • Laser Therapy / adverse effects*
  • Photography
  • Spectrometry, Fluorescence / methods*
  • Tendon Injuries / etiology*
  • Tendon Injuries / pathology*

Substances

  • Azo Compounds
  • Coloring Agents
  • C.I. direct red 80