Erratum

Genesis. 2000 Jul;27(3):136.

Abstract

"Mesenchymal-Epithelial Transition in the Developing Metanephric Kidney: Gene Expression Study by Differential Display," by Sergei Y. Plisov, Sergey V. Ivanov, Kiyoshi Yoshino, Lee F. Dove, Tatiana M. Plisova, Kathleen G. Higinbotham, Irina Karavanova, Michael Lerman, and Alan O. Perantoni The above article originally appeared in Volume 27, Number 1, the May 2000 issue, of genesis on pp. 22-31. The wrong affiliations were listed for two of the co-authors: Sergey V. Ivanov is affiliated with Intramural Research Support Program, SAIC-Frederick, Laboratory of Immunobiology, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland. Michael Lerman is affiliated with Laboratory of Immunobiology, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland. On page 24, left column, under "In situ mRNA Hybridization" in the "Results" section, the sentence, "To verify the results of DD and to determine in which cells of the developing kidney the differentially displayed genes were expressed we applied mRNA hybridization (ISH)," should read: "To verify the results of DD and to determine in which cells of the developing kidney the differentially displayed genes were expressed we applied in situ mRNA hybridization (ISH)." On page 27, the legend for Figure 2, should read: "In situ RNA hybridization with thin sections of 19 dpc fetal kidney. Labeled antisense RNA was in vitro transcribed from cloned cDNA fragments obtained after differential display." On page 30, right column, under "In situ Hybridization" in the "Methods" section, the sentence, "To generate sense or antisense probes, 5 &mgr;g of plasmids with cloned cDNA fragments were linearized either with NcoI or SpeI (Promega) restriction enzymes and transcribed with T7 or SP6 RNA polymerase in the presence of alpha-35S-dCTP," should read: "To generate sense or antisense probes, 5 &mgr;g of plasmids with cloned cDNA fragments were linearized either with NcoI or SpeI (Promega) restriction enzymes and transcribed with T7 or SP6 RNA polymerase in the presence of alpha-35S-CTP." The authors regret these errors.