In vitro reversion of amphotericin B resistance in Leishmania donovani by poloxamer 188

S Espuelas; P Legrand; P M Loiseau; C Bories; G Barratt; J M Irache

doi:10.1128/AAC.44.8.2190-2192.2000

In vitro reversion of amphotericin B resistance in Leishmania donovani by poloxamer 188

Antimicrob Agents Chemother. 2000 Aug;44(8):2190-2. doi: 10.1128/AAC.44.8.2190-2192.2000.

Authors

S Espuelas¹, P Legrand, P M Loiseau, C Bories, G Barratt, J M Irache

Affiliation

¹ Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Navarra, 31080 Pamplona, Spain.

Abstract

A micellar formulation of amphotericin B (AmB) solubilized with poloxamer 188 was evaluated against an AmB Leishmania donovani-resistant line. A concave isobologram showed a synergistic effect of this association against promastigotes. This result was confirmed with amastigotes since the 50% effective concentration of the new formulation was 100 times less than that of the control AmB formulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amphotericin B / pharmacology*
Animals
Antiprotozoal Agents / pharmacology*
Betaine / analogs & derivatives*
Betaine / pharmacology
Drug Evaluation, Preclinical
Drug Resistance, Microbial
Drug Synergism
Inhibitory Concentration 50
Leishmania donovani / drug effects*
Microbial Sensitivity Tests
Poloxamer / pharmacology*

Substances

Antiprotozoal Agents
Poloxamer
desintegron B
Betaine
Amphotericin B