The ability of the API Candida system (bioMérieux, France) to identify Candida lusitaniae isolates was evaluated in comparison to the Auxacolor and ID 32C systems using 52 clinical isolates previously identified on the basis of their morphology and their biochemical reactions in the Auxacolor and ID 32C systems. The API Candida system failed to definitively identify most of the strains tested within 24 h. No beta-maltosidase activity was detected in 28 strains, and supplementary tests were required to discriminate Candida lusitaniae, Candida famata and Candida guilliermondii. The API Candida system is not suitable for identification of Candida lusitaniae. In comparison, the Auxacolor system is easy to use and interpret, allowing rapid identification of this species; however, the ID 32C system is required for identification of atypical strains.