Eremopyrum is a potential genus in wheat improvement. To breed T. aestivum-Er. orientale additional and substitutional lines, chromosome number of 96 BC2F3 individuals were accounted. Fifteen plants with 2n = 43 and eight plants with 2n = 44 were checked out. GISH results of the individuals with 43 chromosomes showed that they blonged to three different monosomic additions. Two disomic additions, one double-monosomic addition, one trible-monosomic addition and one monosomic addition were found in the 2n = 44 individuals. One double-monosomic substitution and one monosomic substitution were obtained. We also proved that it is relatively easier to obtain addition lines in the selfing population than to obtain double-monosomic additions.