[The diploid of genetically engineered yeast for expression of hybrid HBsAg gene SA-28]

X K Chen; H Y Yuan; H Pan; X D Wu; Y Y Li

[The diploid of genetically engineered yeast for expression of hybrid HBsAg gene SA-28]

Sheng Wu Gong Cheng Xue Bao. 2000 Jan;16(1):10-2.

[Article in Chinese]

Authors

X K Chen¹, H Y Yuan, H Pan, X D Wu, Y Y Li

Affiliation

¹ Institute of Genetics, Fudan University, Shanghai.

PMID: 10883267

Abstract

By mating a yeast haploid expressing hybrid HBsAg gene SA-28, Y19/YFD158, with another hapolid Y95, the diploid Y95xY19/YFD158 was constructed. The experiments showed that the cell density of the diploid in fermentation was three time higher than that of the haploid, the stability of expression vector in the diploid was much higher than in the haploid, the xpression level of hybrid HBsAg gene of the diploid was more than three time higher than that of the haploid and the average cope number in diploid cell was a little lower than that in the haploid.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Diploidy*
Gene Expression
Genetic Engineering
Hepatitis B Surface Antigens / genetics*
Yeasts / genetics*

Substances

Hepatitis B Surface Antigens