NC-hybird D2-CA8057 with D2-type cytoplasm and the nucleus of common wheat CA8057, cytoplasmic male-sterile (CMS) line msD2-CA8057 resulting from the variation of D2-type cytoplasm and CMS line msA-CA8057 resulting from the variation of CA8057 cytoplasm were bred. The genetic characters of the four isonucleus-alloplasmic line were studied, and the main results are as follows: (1) there were good genetic effects of cytoplasms on productive and qualitative characters of common wheat, the alloplasmic lines had the potential of NC-heterosis. (2) CMS line msD2-CA8057 and msA-CA8057 had different degenerative characters of anthers and pollen grains, msD2-CA8057 degenerated from the later uninucleus-pollen stage to the binucleus-pollen stage and the pollen grains had starch grains, msA-CA8057 degenerated at the later uninucleus-pollen stage and the pollen grains had no starch grains. (3) msD2-CA8057 and msA-CA8057 were easily maintained but different in the fertility behaviors, the former had wider restoring sources and high digree of restoing, the latter had few restoring sources but there were strains with high restoring ability. The application value of alloplasmic lines and the two new CMS-patterns were evaluated from various aspects, the possible reasons of cytoplasmic variation and the relativity between degenerative pattern of pollen grain and the fertility behavior were discussed.