Hispanic women have higher parity and shorter interbirth intervals than women of other ethnic groups. Thus, they are more likely to become pregnant relatively soon after giving birth, which may place these women at risk of low or deficient levels of specific nutrients. Folic acid is of particular concern because recent studies suggest that maternal use of folic acid supplements may be associated with better reproductive outcomes. The purpose of this study was to assess folic acid levels in postpartum Hispanic women. Using a cross-sectional design, we measured erythrocyte folate values for 188 low-income Hispanic women 1-12 months postpartum who were receiving services at the Women, Infants, and Children (WIC) clinics in El Paso, Texas. An interview was administered to collect information on diet, vitamin use, and method of infant feeding. Mean erythrocyte folate levels decreased from >1300 ng/ml during the first 4 months postpartum to a low of 1017 ng/ml by 12 months postpartum, for an overall decrease of approximately 23% (p = 0.004). Use of postpartum vitamin supplements was significantly associated with higher folate levels. However, only 35% of mothers used vitamins beyond 1 month postpartum. Study results suggest that these mothers may be at risk of developing low or deficient levels of folic acid during the postpartum period. Educational campaigns targeting these women as well as other groups of postpartum women should encourage them to comply with the U.S. Public Health Service recommendation that women of childbearing age consume 0.4 mg of folic acid daily.