Abstract
Adenovirus late mRNA export is facilitated by viral early proteins of 55 and 34 kDa. The 34-kDa protein contains a leucine-rich nuclear export signal (NES) similar to that of the human immunodeficiency virus Rev protein. It was proposed that the 34-kDa protein might facilitate the export of adenovirus late mRNA through a Rev-like NES-mediated export pathway. We have tested the role of NES-mediated RNA export during adenovirus infection, and we find that it is not essential for the expression of adenovirus late genes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / genetics
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Adenoviridae / metabolism
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Adenoviridae / physiology*
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Carrier Proteins / metabolism
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Cell Fusion
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Cell Nucleus / metabolism
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Cycloheximide / pharmacology
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Dactinomycin / pharmacology
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Exportin 1 Protein
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Fatty Acids, Unsaturated / pharmacology
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Gene Products, rev / metabolism*
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HeLa Cells
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Humans
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Karyopherins*
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Mutation
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Nuclear Proteins / metabolism
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Nucleic Acid Synthesis Inhibitors / pharmacology
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Polyethylene Glycols / pharmacology
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Protein Synthesis Inhibitors / pharmacology
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RNA, Viral / metabolism*
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Receptors, Cytoplasmic and Nuclear*
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Surface-Active Agents / pharmacology
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Transcription, Genetic
Substances
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Carrier Proteins
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Fatty Acids, Unsaturated
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Gene Products, rev
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Karyopherins
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Nuclear Proteins
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Nucleic Acid Synthesis Inhibitors
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Protein Synthesis Inhibitors
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RNA, Viral
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Receptors, Cytoplasmic and Nuclear
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Surface-Active Agents
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Dactinomycin
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Polyethylene Glycols
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Cycloheximide
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leptomycin B