Rapid determination of chlorogenic acid and related compounds in sunflower seeds by high-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry

M A Aramendía; I M García; F Lafont; J Lizaso; J M Marinas; F J Urbano

doi:10.1002/1097-0231(20000630)14:12<1019::AID-RCM981>3.0.CO;2-9

Rapid determination of chlorogenic acid and related compounds in sunflower seeds by high-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry

Rapid Commun Mass Spectrom. 2000;14(12):1019-22. doi: 10.1002/1097-0231(20000630)14:12<1019::AID-RCM981>3.0.CO;2-9.

Authors

M A Aramendía¹, I M García, F Lafont, J Lizaso, J M Marinas, F J Urbano

Affiliation

¹ S.C.A.I.-Mass Spectrometry and Organic Chemistry Department, Faculty of Sciences, University of Córdoba, Av. S. Alberto Magno s/n, E-14004 Cordoba, Spain.

PMID: 10861981
DOI: 10.1002/1097-0231(20000630)14:12<1019::AID-RCM981>3.0.CO;2-9

Abstract

High-performance liquid chromatography (HPLC) in combination with atmospheric pressure chemical ionization mass spectrometry (APcI-MS) was applied to the determination of the phenolic fraction found in methanolic extracts of sunflower seeds (mainly chlorogenic acid and derived compounds). These extracts were directly separated by HPLC and detected by both negative and positive APcI-MS. Abundant structural information about these compounds can be obtained even at low extraction cone voltages. This method has been shown to be a rapid and effective method for the analysis of crude extracts from sunflower seeds.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Atmospheric Pressure
Chlorogenic Acid / analogs & derivatives
Chlorogenic Acid / analysis*
Chromatography, High Pressure Liquid / methods
Helianthus / chemistry*
Mass Spectrometry / methods*
Seeds / chemistry*

Substances

Chlorogenic Acid