Major and 5S ribosomal sequences of the largemouth bass Micropterus salmoides (Perciformes, Centrarchidae) are localized in GC-rich regions of the genome

Chromosome Res. 2000;8(3):213-8. doi: 10.1023/a:1009248828365.

Abstract

Major and 5S ribosomal genes have been localized in the chromosomes of Micropterus salmoides. By C-banding, Ag-staining, CMA3-staining and 45S and 5S fluorescence in-situ hybridization (FISH), we demonstrate that the 45S and 5S ribosomal genes are clustered in two different chromosome pairs and both are located in heterochromatic GC-rich regions. PCR amplification and sequencing of the 5S intergenic non-transcribed sequences have allowed us to identify variability essentially due to a trinucleotide tandem repeat (GCT).

MeSH terms

  • Animals
  • Base Sequence
  • Bass / genetics*
  • Chromosome Banding
  • DNA, Ribosomal / genetics*
  • Female
  • Fluorescent Dyes
  • GC Rich Sequence
  • Genome*
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Male
  • Molecular Sequence Data
  • Physical Chromosome Mapping
  • RNA, Ribosomal, 5S / genetics*
  • Sequence Alignment

Substances

  • DNA, Ribosomal
  • Fluorescent Dyes
  • RNA, Ribosomal, 5S