Sperm-mediated preparation of transgenic Xenopus laevis and transmission of transgenic DNA to the next generation

Mol Reprod Dev. 2000 Jun;56(2 Suppl):298-300. doi: 10.1002/(SICI)1098-2795(200006)56:2+<298::AID-MRD19>3.0.CO;2-Q.

Abstract

Sperm-mediated transgenesis of frogs Xenopus laevis with retroviral Rous sarcoma virus DNA is described and the transgenic progeny is characterized. Correlation between the high expression of src gene and defective morphogenesis of frog embryos is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Avian Sarcoma Viruses / genetics
  • Blotting, Southern
  • DNA, Viral / genetics
  • Female
  • Fertilization in Vitro
  • Gene Transfer Techniques*
  • In Vitro Techniques
  • Male
  • Ovum / cytology
  • Polymerase Chain Reaction
  • Spermatozoa / cytology*
  • Transgenes*
  • Xenopus laevis / genetics*
  • src-Family Kinases / genetics
  • src-Family Kinases / metabolism

Substances

  • DNA, Viral
  • src-Family Kinases