Abstract
The bovine leukemia virus (BLV) envelope gene encoding extracellular glycoprotein gp51 and transmembrane glycoprotein gp30 was cloned into a vehicle expression vector under the human cytomegalovirus (CMV) intermediate early promoter. The intramuscular injection of this plasmid vector generated a cellular immune response. Seven out of ten cows vaccinated with the DNA construct resisted a drastic challenge (500 BLV-infected lymphocytes as an infectious dose).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis
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Base Sequence
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Cattle
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DNA Primers / genetics
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Enzootic Bovine Leukosis / immunology
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Enzootic Bovine Leukosis / prevention & control*
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Genes, env
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Humans
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Immunity, Cellular
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Leukemia Virus, Bovine / genetics*
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Leukemia Virus, Bovine / immunology*
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Plasmids / genetics
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / pharmacology*
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Viral Vaccines / administration & dosage
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Viral Vaccines / pharmacology*
Substances
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Antibodies, Viral
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DNA Primers
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Vaccines, DNA
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Viral Vaccines