Tachykinin NK(2) receptors facilitate acetylcholine release from guinea-pig isolated trachea

Eur J Pharmacol. 2000 May 12;396(1):29-32. doi: 10.1016/s0014-2999(00)00199-0.

Abstract

The release of newly synthesised [3H]acetylcholine was evoked by electrical field stimulation (5 Hz, 600 pulses) of epithelium-deprived guinea-pig trachea strips after sensory neuropeptides depletion with 3 microM capsaicin. The selective tachykinin NK(2) receptor agonist [betaAla(8)]neurokinin A-(4-10) increased in a concentration-dependent manner the electrically-induced release of [3H]acetylcholine. The facilitatory effect was antagonised by the selective non-peptide tachykinin NK(2) receptor antagonist, SR 48968 (apparent pK(B) 8.9). The tachykinin NK(1) and NK(3) receptor agonists substance P methyl ester and senktide (both 10 and 100 nM), respectively, did not affect the evoked release of [3H]acetylcholine. It is concluded that the cholinergic nerves of guinea-pig trachea are endowed with prejunctional facilitatory tachykinin receptors of the NK(2) subtype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / metabolism*
  • Animals
  • Benzamides / pharmacology
  • Capsaicin / pharmacology
  • Choline / metabolism
  • Electric Stimulation
  • Guinea Pigs
  • In Vitro Techniques
  • Male
  • Neurokinin A / analogs & derivatives
  • Neurokinin A / pharmacology
  • Neurons / drug effects
  • Neurons / metabolism
  • Peptide Fragments / pharmacology
  • Piperidines / pharmacology
  • Receptors, Neurokinin-2 / agonists
  • Receptors, Neurokinin-2 / antagonists & inhibitors
  • Receptors, Neurokinin-2 / drug effects*
  • Trachea / drug effects
  • Trachea / metabolism*

Substances

  • Benzamides
  • Peptide Fragments
  • Piperidines
  • Receptors, Neurokinin-2
  • neurokinin A (4-10), beta-Ala(8)-
  • SR 48968
  • Neurokinin A
  • Choline
  • Acetylcholine
  • Capsaicin