Inactivation of the glutamate decarboxylase gene in Lactococcus lactis subsp. cremoris

Appl Environ Microbiol. 2000 May;66(5):2235-7. doi: 10.1128/AEM.66.5.2235-2237.2000.

Abstract

Lactococcus lactis subsp. lactis strains show glutamate decarboxylase activity, whereas L. lactis subsp. cremoris strains do not. The gadB gene encoding glutamate decarboxylase was detected in the L. lactis subsp. cremoris genome but was poorly expressed. Sequence analysis showed that the gene is inactivated by the frameshift mutation and encoded in a nonfunctional protein.

Publication types

  • Comparative Study

MeSH terms

  • Base Sequence
  • Frameshift Mutation*
  • Glutamate Decarboxylase / genetics*
  • Lactococcus lactis / classification
  • Lactococcus lactis / enzymology*
  • Lactococcus lactis / genetics*
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid

Substances

  • Glutamate Decarboxylase

Associated data

  • GENBANK/AB033218
  • GENBANK/AB033230