Synthesis of oligonucleotides containing the oxidatively modified kappaB site: unique binding affinity to the NFkappaB p50 homodimer

A Kittaka; H Takayama; C Horii; T Kuze; H Tanaka; K T Nakamura; T Miyasaka; J Inoue

doi:10.1093/nass/42.1.33

Synthesis of oligonucleotides containing the oxidatively modified kappaB site: unique binding affinity to the NFkappaB p50 homodimer

Nucleic Acids Symp Ser. 1999:(42):33-4. doi: 10.1093/nass/42.1.33.

Authors

A Kittaka¹, H Takayama, C Horii, T Kuze, H Tanaka, K T Nakamura, T Miyasaka, J Inoue

Affiliation

¹ Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa, Japan.

PMID: 10780365
DOI: 10.1093/nass/42.1.33

Abstract

Synthesis of oligonucleotide 26-mers including single 5-formyl-2'-deoxyuridine (1) or 5-formyl-2'-O-methyluridine (2) in place of thymidine at the kappaB site has been accomplished. One of the 26-mers with 1 was critically discriminated by the NFkappaB p50 homodimer in binding.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Binding Sites
DNA / chemistry
Dimerization
NF-kappa B / chemistry*
NF-kappa B / metabolism*
Oligodeoxyribonucleotides / chemical synthesis*
Oligodeoxyribonucleotides / chemistry*
Oxidation-Reduction

Substances

NF-kappa B
Oligodeoxyribonucleotides
DNA