Characterization of a novel transcription factor binding to the regulatory regions of the human pro-alpha1(I) collagen gene

Arch Biochem Biophys. 2000 Apr 15;376(2):281-7. doi: 10.1006/abbi.2000.1759.

Abstract

We have identified a novel transcription factor TGP (TG Binding Protein) that binds to the consensus sequence 5'-TGTGGGGTGG-3' in the promoter and intron of the human pro-alpha1(I) collagen gene. This recognition sequence, or sequences closely resembling these sequences, was also identified in the pro-alpha1(I) and pro-alpha2(I) collagen genes of other species. Competition experiments revealed that TGP is related to but distinguishable from the Ap4/5 family of transcription factors and that it can be separated from Ap4/5 according to size.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding, Competitive
  • Cell Line
  • Consensus Sequence / genetics
  • DNA / genetics
  • DNA / metabolism
  • DNA Footprinting
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism*
  • Enhancer Elements, Genetic / genetics
  • Humans
  • Introns / genetics
  • Molecular Weight
  • Oligodeoxyribonucleotides / genetics
  • Oligodeoxyribonucleotides / metabolism
  • Procollagen / genetics*
  • Promoter Regions, Genetic / genetics*
  • Response Elements / genetics*
  • Species Specificity
  • Substrate Specificity
  • Trans-Activators / chemistry
  • Trans-Activators / metabolism
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism*

Substances

  • DNA-Binding Proteins
  • Oligodeoxyribonucleotides
  • Procollagen
  • Trans-Activators
  • Transcription Factors
  • enhancer-binding protein AP-4
  • transcription factor AP-5
  • DNA