Abstract
We have identified a novel transcription factor TGP (TG Binding Protein) that binds to the consensus sequence 5'-TGTGGGGTGG-3' in the promoter and intron of the human pro-alpha1(I) collagen gene. This recognition sequence, or sequences closely resembling these sequences, was also identified in the pro-alpha1(I) and pro-alpha2(I) collagen genes of other species. Competition experiments revealed that TGP is related to but distinguishable from the Ap4/5 family of transcription factors and that it can be separated from Ap4/5 according to size.
Copyright 2000 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding, Competitive
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Cell Line
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Consensus Sequence / genetics
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DNA / genetics
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DNA / metabolism
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DNA Footprinting
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism*
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Enhancer Elements, Genetic / genetics
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Humans
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Introns / genetics
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Molecular Weight
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Oligodeoxyribonucleotides / genetics
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Oligodeoxyribonucleotides / metabolism
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Procollagen / genetics*
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Promoter Regions, Genetic / genetics*
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Response Elements / genetics*
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Species Specificity
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Substrate Specificity
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Trans-Activators / chemistry
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Trans-Activators / metabolism
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Transcription Factors / chemistry
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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Oligodeoxyribonucleotides
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Procollagen
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Trans-Activators
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Transcription Factors
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enhancer-binding protein AP-4
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transcription factor AP-5
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DNA