Abstract
We present a novel method for direct introduction of chemicals into a sieve tube of intact rice plants-namely, the method of micro-introduction using stylet of insect (the MUSI method). Fluorescent dyes were successfully introduced into a sieve tube through a severed stylet of planthopper and the distribution of the dyes was observed.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Fluorescein-5-isothiocyanate / administration & dosage
-
Fluorescein-5-isothiocyanate / pharmacokinetics
-
Fluorescent Dyes / administration & dosage
-
Fluorescent Dyes / pharmacokinetics*
-
Insecta
-
Microinjections / methods
-
Microscopy, Fluorescence
-
Oryza / metabolism*
-
Plant Leaves / metabolism*
-
Rhodamines / administration & dosage
-
Rhodamines / pharmacokinetics
Substances
-
Fluorescent Dyes
-
Rhodamines
-
tetramethylrhodamine
-
Fluorescein-5-isothiocyanate