A new method for direct introduction of chemicals into a single sieve tube of intact rice plants

Plant Cell Physiol. 2000 Jan;41(1):124-8. doi: 10.1093/pcp/41.1.124.

Abstract

We present a novel method for direct introduction of chemicals into a sieve tube of intact rice plants-namely, the method of micro-introduction using stylet of insect (the MUSI method). Fluorescent dyes were successfully introduced into a sieve tube through a severed stylet of planthopper and the distribution of the dyes was observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fluorescein-5-isothiocyanate / administration & dosage
  • Fluorescein-5-isothiocyanate / pharmacokinetics
  • Fluorescent Dyes / administration & dosage
  • Fluorescent Dyes / pharmacokinetics*
  • Insecta
  • Microinjections / methods
  • Microscopy, Fluorescence
  • Oryza / metabolism*
  • Plant Leaves / metabolism*
  • Rhodamines / administration & dosage
  • Rhodamines / pharmacokinetics

Substances

  • Fluorescent Dyes
  • Rhodamines
  • tetramethylrhodamine
  • Fluorescein-5-isothiocyanate