Abstract
The androgen receptor (AR), like other steroid receptors, modulates the activity of the general transcription machinery on the core promoter to exert its function as a regulator. Co-immunoprecipitation of prostate cancer LNCaP cell extract using protein A-Sepharose coupled with anti-AR antibody indicates that the AR interacts with the general transcription factor TFIIH in a physiological condition. Co-transfection of cdk activating kinase (CAK), the kinase moiety of TFIIH, enhanced AR-mediated transcription in a ligand-dependent manner in human prostate cancer PC-3 and LNCaP cells, and in a ligand-independent manner in human prostate cancer DU145 cells. Detailed interaction studies further revealed that the AR NH(2)-terminal domain interacting with CAK was essential for the CAK-induced AR transactivation. Together, our data suggest that the AR may interact with TFIIH for efficient communication with the general transcription factors/RNA polymerase II on the core promoter.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cyclin H
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Cyclin-Dependent Kinase-Activating Kinase
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Cyclin-Dependent Kinases*
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Cyclins / metabolism
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DNA-Binding Proteins
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Humans
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Male
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Prostatic Neoplasms / metabolism
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Prostatic Neoplasms / pathology
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Protein Serine-Threonine Kinases / chemistry
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Protein Serine-Threonine Kinases / metabolism*
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Receptors, Androgen / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae Proteins*
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TATA-Binding Protein Associated Factors*
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Transcription Factor TFIID*
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Transcription Factor TFIIH
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription Factors, TFII*
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Tumor Cells, Cultured
Substances
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CCNH protein, human
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Cyclin H
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Cyclins
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DNA-Binding Proteins
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Fungal Proteins
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GAL4 protein, S cerevisiae
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Receptors, Androgen
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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TAF6 protein, S cerevisiae
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TATA-Binding Protein Associated Factors
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Transcription Factor TFIID
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Transcription Factors
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Transcription Factors, TFII
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Transcription Factor TFIIH
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Protein Serine-Threonine Kinases
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Cyclin-Dependent Kinases
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Cyclin-Dependent Kinase-Activating Kinase