Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach

Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4309-14. doi: 10.1073/pnas.070525997.

Abstract

We propose a rational approach to the generation of live viral vaccines: alteration of virally encoded type I IFN antagonists to attenuate virulence while retaining immunogenicity. We have explored this concept by using the influenza virus. Previously we have shown that the NS1 protein of influenza A virus possesses anti-IFN activity. We now present evidence that influenza A and B viruses encoding altered viral NS1 proteins are highly attenuated in the mouse host, yet provide protection from challenge with wild-type viruses.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Chlorocebus aethiops
  • DNA Primers
  • Dogs
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Influenza A virus / genetics*
  • Influenza A virus / immunology
  • Influenza B virus / genetics*
  • Influenza B virus / immunology
  • Influenza Vaccines / genetics*
  • Influenza Vaccines / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mutagenesis, Site-Directed
  • Vero Cells
  • Viral Nonstructural Proteins / genetics*
  • Viral Nonstructural Proteins / immunology

Substances

  • DNA Primers
  • INS1 protein, influenza virus
  • Influenza Vaccines
  • Viral Nonstructural Proteins