Large scale, liquid phase oligonucleotide synthesis by alkyl H-phosphonate approach

K J Padiya; M M Salunkhe

doi:10.1016/s0968-0896(99)00287-4

Large scale, liquid phase oligonucleotide synthesis by alkyl H-phosphonate approach

Bioorg Med Chem. 2000 Feb;8(2):337-42. doi: 10.1016/s0968-0896(99)00287-4.

Authors

K J Padiya¹, M M Salunkhe

Affiliation

¹ Department of Chemistry, The Institute of Science, Mumbai, India.

PMID: 10722156
DOI: 10.1016/s0968-0896(99)00287-4

Abstract

A new approach to the liquid phase synthesis of oligonucleotide is described, it is based on oxidative coupling using alkyl H-phosphonate synthon and polyethylene glycol (PEG5000) as a soluble support. Nucleoside alkyl H-phosphonate undergoes oxidative coupling in presence of NBS. The use of polyethylene glycol as a soluble polymeric support preserves some convenient features of the solid phase synthesis with new interesting advantages. This liquid phase method appears effective in terms of speed and coupling yield and can be evaluated for the production of large amount of oligonucleotide (100 microM).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Magnetic Resonance Spectroscopy
Oligonucleotides / chemical synthesis*
Organophosphonates / chemistry*
Oxidation-Reduction

Substances

Oligonucleotides
Organophosphonates