Objective: To elucidate the inhibited effect and regulated mechanism of IGFBp-1 on the carcinoma cells.
Methods: The recombinant human IGF II (rhIGF II), phosphorylated and nonphosphorylated binding protein-1(pBp-1 and npBp-1) that were purified from pregnant woman's amnoitic fluid were used to cultivate cancer cells from 15 cases with hepatocellular carcinoma and ascites. The cell counting and MTT methods were used for determining the dynamics change of proliferation on HCC, and the in situ or dot hybridization with Digoxin label used for the expression of IGF II mRNA qualitatively and quantitatively at different times.
Results: The cells grew more slowly, in pBp-1 group, than in control group at the same time, which began to decrease at two days(P < 0.05) by MTT. In situ hybridization showed that there are hybridization signal of IGF II mRNA in all groups during the culture, and scanning dot hybridization showed the OPTMD of IGF II mRNA in pBp-1 group was statistically different from control and other groups (P < 0.01), while in npBp-1 group there was no significant effect compared with other groups (P > 0.05).
Conclusion: The pBp-1 can inhibit the growth of hepatoma cells and the expression of IGF II mRNA, which may be regulated and inhibited by pBp-1.