Structure of the O-specific polysaccharide of the bacterium Proteus mirabilis O29

A V Perepelov; A S Shashkov; S N Senchenkova; Y A Knirel; E Literacka; W Kaca

Structure of the O-specific polysaccharide of the bacterium Proteus mirabilis O29

Biochemistry (Mosc). 2000 Feb;65(2):176-9.

Authors

A V Perepelov¹, A S Shashkov, S N Senchenkova, Y A Knirel, E Literacka, W Kaca

Affiliation

¹ Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia.

PMID: 10713543

Abstract

An O-specific polysaccharide was obtained by mild acid degradation of P. mirabilis O29 lipopolysaccharide (LPS) and found to contain 2-acetamido-2-deoxy-D-galactose and D-glucuronic acid (D-GlcA) in the ratio 3:1. Studies of the polysaccharide by 1H- and 13C-NMR spectroscopy including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), and H-detected 1H,13C-heteronuclear multiple-quantum coherence (HMQC) experiments demonstrated the following structure of the branched tetrasaccharide repeating unit:

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens / chemistry*
O Antigens / isolation & purification
Proteus mirabilis / chemistry*
Proteus mirabilis / immunology

Substances

O Antigens