Cloning and characterization of cDNA encoding zebrafish Danio rerio NM23-B gene

Gene. 2000 Mar 7;245(1):75-9. doi: 10.1016/s0378-1119(00)00037-8.

Abstract

A full-length zebrafish NM23-B cDNA was cloned and sequenced. The zebrafish NM23-B cDNA consists of 624bp with an open reading frame of 153 amino acids. NM23-B mRNA of approximately 0.7kb is present in adult zebrafish tissues. Zebrafish NM23-B his-tagged protein (17kDa) was produced in E. coli and characterized by binding and UV-cross-linking to a single-stranded telomeric repeat (TTAGGG)(6). This is the first report to show that fish have a NM23-H2 homologue that is similar to that in humans.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Cross-Linking Reagents
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics*
  • DNA, Single-Stranded / genetics
  • DNA, Single-Stranded / metabolism
  • Female
  • Gene Expression
  • Molecular Sequence Data
  • Monomeric GTP-Binding Proteins / genetics*
  • Monomeric GTP-Binding Proteins / metabolism
  • NM23 Nucleoside Diphosphate Kinases
  • Nucleoside-Diphosphate Kinase*
  • Oligonucleotides / genetics
  • Oligonucleotides / metabolism
  • Protein Binding
  • RNA / genetics
  • RNA / metabolism
  • Repetitive Sequences, Nucleic Acid
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Telomere / genetics
  • Telomere / metabolism
  • Tissue Distribution
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Ultraviolet Rays
  • Zebrafish / genetics*

Substances

  • Cross-Linking Reagents
  • DNA, Complementary
  • DNA, Single-Stranded
  • NM23 Nucleoside Diphosphate Kinases
  • Oligonucleotides
  • Transcription Factors
  • RNA
  • Nucleoside-Diphosphate Kinase
  • Monomeric GTP-Binding Proteins

Associated data

  • GENBANK/AF201764