Abstract
A full-length zebrafish NM23-B cDNA was cloned and sequenced. The zebrafish NM23-B cDNA consists of 624bp with an open reading frame of 153 amino acids. NM23-B mRNA of approximately 0.7kb is present in adult zebrafish tissues. Zebrafish NM23-B his-tagged protein (17kDa) was produced in E. coli and characterized by binding and UV-cross-linking to a single-stranded telomeric repeat (TTAGGG)(6). This is the first report to show that fish have a NM23-H2 homologue that is similar to that in humans.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Blotting, Northern
-
Cloning, Molecular
-
Cross-Linking Reagents
-
DNA, Complementary / chemistry
-
DNA, Complementary / genetics*
-
DNA, Single-Stranded / genetics
-
DNA, Single-Stranded / metabolism
-
Female
-
Gene Expression
-
Molecular Sequence Data
-
Monomeric GTP-Binding Proteins / genetics*
-
Monomeric GTP-Binding Proteins / metabolism
-
NM23 Nucleoside Diphosphate Kinases
-
Nucleoside-Diphosphate Kinase*
-
Oligonucleotides / genetics
-
Oligonucleotides / metabolism
-
Protein Binding
-
RNA / genetics
-
RNA / metabolism
-
Repetitive Sequences, Nucleic Acid
-
Sequence Alignment
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
-
Telomere / genetics
-
Telomere / metabolism
-
Tissue Distribution
-
Transcription Factors / genetics*
-
Transcription Factors / metabolism
-
Ultraviolet Rays
-
Zebrafish / genetics*
Substances
-
Cross-Linking Reagents
-
DNA, Complementary
-
DNA, Single-Stranded
-
NM23 Nucleoside Diphosphate Kinases
-
Oligonucleotides
-
Transcription Factors
-
RNA
-
Nucleoside-Diphosphate Kinase
-
Monomeric GTP-Binding Proteins