Structure, measurement, and secretion of human glucagon-like peptide-2

Peptides. 2000 Jan;21(1):73-80. doi: 10.1016/s0196-9781(99)00176-x.

Abstract

By using radioimmunoassays toward the cDNA-predicted amino acid sequence of human glucagon-like peptide-2, a peptide was isolated from extracts of human ileum. By mass spectrometry and Edman sequencing, this peptide was identified as human proglucagon 126-158. High-performance liquid chromatography analyses indicated that a similar immunoreactive peptide (iGLP-2) was present in human plasma. Human plasma concentrations of iGLP-2 were elevated 3- to 4-fold at 1 to 2 h after ingestion of 800 to 1200 kcal meals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Glucagon / analysis
  • Glucagon / chemistry
  • Glucagon / metabolism
  • Glucagon-Like Peptide 2
  • Glucagon-Like Peptides
  • Humans
  • Ileum / chemistry
  • Mass Spectrometry
  • Pancreas / chemistry
  • Peptide Fragments / analysis
  • Peptide Fragments / chemistry
  • Peptide Fragments / metabolism
  • Peptides / analysis
  • Peptides / chemistry*
  • Peptides / metabolism
  • Proglucagon
  • Protein Precursors / analysis
  • Protein Precursors / chemistry
  • Protein Precursors / metabolism
  • Radioimmunoassay / methods

Substances

  • Glucagon-Like Peptide 2
  • Peptide Fragments
  • Peptides
  • Protein Precursors
  • Proglucagon
  • Glucagon-Like Peptides
  • Glucagon