Comparative studies on detecting CAg in urine of acute schistosomiasis patients by mAb-RIHA and mAb-DotELISA

Southeast Asian J Trop Med Public Health. 1999 Mar;30(1):29-31.

Abstract

Urine was concentrated 20-fold for assay for CAg of Schistosoma japonicum. mAb-RIHA and mAb-DotELISA were positive in 78, 31% and 65.06% of cases respectively, of 83 patients with acute schistosomiasis. The false positive rates in 101 healthy controls were 14.85% and 0%, respectively. Cross-reactions (using mAb-RIHA) were seen in 16.36% and 14.28% of patients with clonorchiasis, 49 patients with ankylostomiasis, respectively. Corresponding figures for mAb-DotELISA were 0% and 0%.

Publication types

  • Comparative Study

MeSH terms

  • Acute Disease
  • Antibodies, Monoclonal*
  • Antigens, Helminth*
  • Case-Control Studies
  • Clonorchiasis / immunology
  • Clonorchiasis / urine
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay / methods*
  • False Positive Reactions
  • Glycoproteins / immunology*
  • Glycoproteins / urine*
  • Helminth Proteins / immunology*
  • Helminth Proteins / urine*
  • Hookworm Infections / immunology
  • Hookworm Infections / urine
  • Humans
  • Reproducibility of Results
  • Schistosomiasis japonica / drug therapy
  • Schistosomiasis japonica / immunology*
  • Schistosomiasis japonica / urine*
  • Sensitivity and Specificity

Substances

  • Antibodies, Monoclonal
  • Antigens, Helminth
  • Glycoproteins
  • Helminth Proteins
  • circulating anodic antigen, Schistosoma mansoni