Cernuosides A and B, two sucrase inhibitors from Pulsatilla cernua

J Nat Prod. 2000 Feb;63(2):276-8. doi: 10.1021/np990207+.

Abstract

Two oleanane-type oligoglycosides, cernuosides A (1) and B (2), were isolated from the roots of Pulsatilla cernua (Ranunculaceae). Structure elucidation was accomplished by 1D and 2D NMR (DQF-COSY, TOCSY, HMQC, HMBC, and ROESY) methods, FABMS, and hydrolysis. Both compounds showed moderate activity against sucrase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbohydrate Sequence
  • China
  • Enzyme Inhibitors / isolation & purification*
  • Enzyme Inhibitors / pharmacology
  • Glycosides / isolation & purification*
  • Glycosides / pharmacology
  • Hydrolysis
  • Intestine, Small / drug effects
  • Intestine, Small / enzymology
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Oleanolic Acid* / analogs & derivatives*
  • Plant Roots / chemistry
  • Plants, Medicinal / chemistry*
  • Rats
  • Spectrometry, Mass, Fast Atom Bombardment
  • Sucrase / antagonists & inhibitors*

Substances

  • Enzyme Inhibitors
  • Glycosides
  • cernuoside A
  • cernuoside B
  • Oleanolic Acid
  • Sucrase