Isolated rat heart was subjected to Langendorff perfusion for 10 minutes of total global ischemia, and reperfusion for 15 minutes. The content of myocardial intracellular calcium, content of oxygen free radical induced lipid peroxide mitabolite-malondialdehyde (MDA), content of Lactic dehydrogenase (LDH) in coronary effluent, and the change of myocardial morphology were studied. The effects of flunarizine(FNZ) and/or vitamin C(Vit C) on the above parameters were observed. The results showed that, at 15 minutes of reperfusion, the contents of myocardial intracellular calcium, of MDA in myocaredium and of LDH in coronary effluent in the FNZ + Vit C group, Vit C group and FNZ group were all lower than those in the control group, and the change of morphology in the three groups was also slighter than that in the control group. When FNZ and Vit C were administered together, the effect was more marked and equivalent to the sum total of the effects of the two drugs. These suggest that both FNZ and Vit C reduce reperfusion injury through suppression of myocardium calcium overload and oxygen free radical damage.