[Effects of acetylcholine on inositol 1,4,5-triphosphate formation in cervix uteri myometrial cells of human]

Zhongguo Yao Li Xue Bao. 1999 Jul;20(7):659-62.
[Article in Chinese]

Abstract

Aim: To detect inositol 1,4,5-triphosphate (IP3) formation of pregnant and nonpregnant human myometrial cells induced by acetylcholine (ACh).

Methods: [3H] IP3 competitive protein binding assay.

Results: Basal levels of IP3 in pregnant and nonpregnant human myometrial cells were (82 +/- 9) and (96 +/- 10) nmol.g-1 (protein), respectively (n = 6). Incubated with ACh 50 mumol.L-1 for 5 min, IP3 reached the peak levels (109 +/- 11) and (122 +/- 15) nmol.g-1 (protein), respectively (n = 6), but difference of the increments of IP3 in pregnant and nonpregnant women was not significant. The increased IP3 induced by ACh was inhibited by atropine (Atr) 1 mumol.L-1.

Conclusion: The basal IP3 level in pregnant cervix myometrial cells was higher than that in nonpregnant women. ACh increased the IP3 formation.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / pharmacology*
  • Adult
  • Atropine / pharmacology
  • Cell Separation
  • Female
  • Humans
  • Inositol 1,4,5-Trisphosphate / biosynthesis*
  • Muscarinic Antagonists / pharmacology
  • Myometrium / cytology
  • Myometrium / metabolism*
  • Oxytocin / pharmacology
  • Pregnancy / metabolism*
  • Vasodilator Agents / pharmacology

Substances

  • Muscarinic Antagonists
  • Vasodilator Agents
  • Oxytocin
  • Atropine
  • Inositol 1,4,5-Trisphosphate
  • Acetylcholine